Sample / Representative Certificate of Analysis — each shipment ships with a batch-specific COA
CHESTNUT SUPPLYB2B WHOLESALE · NORCROSS, GA · USA
CERTIFICATE OF ANALYSIS
Document: COA-SAMPLE · Rev. 1
ProductBlack Seed Oil (cold-pressed)
Batch / Lot No.SAMPLE-0000
Botanical nameNigella sativa L.
Production date—— / —— / ——
Also known asBlack Cumin Seed Oil · Kalonji Oil
Best before24 months from production
INCINigella Sativa Seed Oil
Country of originTürkiye
GradeFood-grade / Edible
ExtractionCold-pressed (mechanical, no solvents)
Active Compound
Parameter
Specification
Result (sample)
Method
Thymoquinone (TQ), standard grade
1.2 – 2.4%
2.3%
UPC² / HPLC
Thymoquinone (TQ), high-potency option
up to ~3.0%
On request
UPC² / HPLC
High-TQ batches verified individually; actual measured TQ stated on each batch-specific COA.
Fatty Acid Profile
Fatty acid
Typical range
Result (sample)
Method
Linoleic acid (C18:2, ω-6)
50 – 60%
56%
GC-MS / GC-FID
Oleic acid (C18:1, ω-9)
20 – 25%
24%
GC-MS / GC-FID
Palmitic acid (C16:0)
10 – 13%
11%
GC-MS / GC-FID
Stearic acid (C18:0)
2 – 4%
3%
GC-MS / GC-FID
Total unsaturated fatty acids
80 – 85%
84%
GC-MS / GC-FID
Physical & Chemical
Parameter
Typical
Result (sample)
Method
Appearance
Clear oily liquid
Clear, fluid
Visual
Color
Amber to dark amber
Dark amber
Visual
Odor
Characteristic
Characteristic
Organoleptic
Free fatty acids (as oleic)
≤ 3.0%
1.4%
Titration
Peroxide value
≤ 10 meq O₂/kg
3.5 meq O₂/kg
Titration
Contaminant & Microbial Panel Panel in progress
Parameter
Specification
Result
Method
Heavy metals — Lead (Pb)
≤ spec
Pending — accredited panel
ICP-MS
Heavy metals — Arsenic / Cadmium / Mercury
≤ spec
Pending — accredited panel
ICP-MS
Pesticide residues
Compliant
Pending — accredited panel
GC-MS/MS
Microbial (TPC, Y&M, E. coli, Salmonella)
Compliant
Pending — accredited panel
USP / AOAC
Contaminant & microbial panel is being added via an accredited laboratory; full results will appear on batch COAs upon completion.
Analysis performed using accredited third-party laboratory methods (GC-MS, UPC²/HPLC). This is a representative
sample document showing the structure and typical specifications of our Certificate of Analysis; it is not a
batch release document. Each shipment is accompanied by a batch-specific COA stating actual measured values for
that lot. This document is not a medical or therapeutic claim and does not certify the entire product line.